Free and confidential HIV testing

HIV Testing Schedule

Trillium Health Main Office

259 Monroe Avenue at Monroe Square
Rochester, NY 14607

Free and confidential HIV testing

Mon/Wed/Fri   9 am - 4:30 pm

Tue/Thu         9 am - 6:30 pm

Hepatitis C testing and STD screening (syphilis, gonorrhea, chlamydia) also available weekdays according to risk/behavior profile.  Please note, STD testing is unavailable 12:40 - 1:40 pm when the lab is closed for lunch.

ASL interpretation for testing is available at the Monroe Ave office by appointment only, please contact Jonathan Jordan at 585-210-4112 or

We also offer free testing at our Health Outreach Project site, 416 Central Avenue, Mon – Fri, 1 pm – 4 pm

Testing methods

  • Rapid HIV: finger stick – results in 20 minutes
  • HIV confirmatory testing: blood draw – results in 7 business days
  • Rapid Hep C: finger stick – results in 20 minute
  • STD: urine collection for gonorrhea & chlamydia screening, blood draw for syphilis screening – results in 3 business days

Directions for main office location

Trillium Health’s main office is located at 259 Monroe Avenue, between Union and Alexander Streets, in Rochester, NY and is less than a quarter-mile from the East End, Center City/Central Business District, South Wedge, and Upper Monroe Neighborhoods.

You can reach our main office on the 7 Monroe RTS Bus Route. The bus will drop you off right in front of the building.

Parking at Monroe Square is free, with spaces located behind the building. Just drive up to the yellow gates and they will open automatically.

Enter the building under the Monroe Square sign, walk down the hall and behind the Security Booth is the Care Management and Testing Services reception window.

Other Trillium Health locations

Health Outreach Project
416 Central Ave.
Rochester, NY 14605
Monday – Friday: Walk-in HIV testing hours 1 pm – 4 pm
English/Spanish spoken

Finger Lakes Office
30 Elm St. 
Geneva, NY 14456
Call for Appointment

Southern Tier Office
122 Liberty St.
Bath, NY 14810
Call for Appointment

Questions? Call us at 585-545-7200