Free and confidential HIV/Hep C/STD testing

Testing Schedule

Each test encounter includes a personalized risk assessment, testing, and risk reduction counseling with a trained HIV test counselor. Referrals to Trillium Health’s clinical, care management, insurance, and PEP/PrEP services are available. Testing encounters take an average of 45 minutes, not including additional connections to care.

Trillium Health Main Office

259 Monroe Avenue at Monroe Square
Rochester, NY 14607

Mon/Wed/Thu/Fri   8 am - 6 pm*

Tue   8 am - 7 pm*

Sat   8 am - 4 pm

*Please note, STD testing is unavailable 12:40 - 1:40 pm when the lab is closed for lunch.

ASL interpreters are available at the Trillium Health Main Office by appointment only.

For additional information about our testing services, please contact Jonathan Jordan at 585-210-4112 or

Free HIV and Hep C testing also available at our Health Outreach Project site, 416 Central Avenue, Mon – Fri, 1 pm – 4 pm

Testing methods

  • Rapid HIV: Alere Determine™HIV-1/2 Ag/Ab Combo, finger stick – same day results
  • HIV confirmatory testing: blood draw at on-site lab – results in 7 business days
  • Rapid Hep C: OraQuick® HCV Rapid Antibody Test, finger stick – same day results
  • STD: urine collection for gonorrhea & chlamydia screening, blood draw at on-site lab for syphilis screening – results in 3 business days

Directions for main office location

Trillium Health’s main office is located at 259 Monroe Avenue, between Union and Alexander Streets, in Rochester, NY and is less than a quarter-mile from the East End, Center City/Central Business District, South Wedge, and Upper Monroe Neighborhoods.

You can reach our main office on the 7 Monroe RTS Bus Route. The bus will drop you off right in front of the building.

Parking at Monroe Square is free, with spaces located behind the building. Just drive up to the yellow gates and they will open automatically.

Enter the building under the Monroe Square sign, walk down the hall and behind the Security Booth is the Care Management and Testing Services reception window.


Other Trillium Health locations

Health Outreach Project 
416 Central Ave.
Rochester, NY 14605
Walk-in HIV and Hep C testing hours: Monday – Friday, 1 pm – 4 pm
English/Spanish spoken 

Finger Lakes Office 
30 Elm St. 
Geneva, NY 14456 
Call for Appointment 

Southern Tier Office 
122 Liberty St. 
Bath, NY 14810 
Call for Appointment